RNA-Seq of Nemopilema nomurai tentacles - SRA

SRX5005072: RNA-Seq of Nemopilema nomurai tentacles
1 BGISEQ (BGISEQ-500) run: 118,243 spots, 71.2M bases, 20Mb downloads

Design: poly(A) mRNA was isolated using Oligo (dT) beads and interrupted to short fragments. These fragments were then transcribed into first-strand cDNA, followed by synthesis of the second strand. The synthesized cDNA products were purified using a QiaQuick PCR extraction kit (Qiagen, Valencia, CA, USA) and dissolved in EB buffer for end repair and poly(A) addition. Subsequently, the cDNA fragments were ligated to the sequencing adapters and subjected to size selection using agarose gel electrophoresis. Suitable fragments were amplified by PCR, and the cDNA library was sequenced using an Illumina HiSeq 2000 sequencer at the Beijing Genomics Institute (BGI; Shenzhen, China).The image data output from the sequencer was transformed into sequence data called raw reads. After filtering low-quality reads and reads containing more than 5% unknown nucleotides, the sequencing adaptors were removed from the raw reads. Subsequently, the raw reads were assembled into contigs and unigenes by de novo assembly, which was performed with the Trinity program

Submitted by: Second Military Medical University

Study: Nemopilema nomurai Assembly

PRJNA505091 • SRP168534 • All experiments • All runs

show Abstracthide Abstract

We performed de novo transcriptome sequencing of the tentacle tissue of the jellyfish Nemopilema nomurai .We obtained a well-categorized and annotated transcriptome of Nemopilema nomurai tentacle that will be an important and valuable resource for further understanding of jellyfish at the molecular level and information on the underlying molecular mechanisms of jellyfish stinging. The findings of this study may also be used in comparative studies of gene expression profiling among different jellyfish species.

Sample: Invertebrate sample from Nemopilema nomurai

SAMN10410785 • SRS4039435 • All experiments • All runs

Organism: Nemopilema nomurai

Library:

Name: Unigene of Nemopilema nomurai tentacles

Instrument: BGISEQ-500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Runs: 1 run, 118,243 spots, 71.2M bases, 20Mb

Run	# of Spots	# of Bases	Size	Published
SRR8185105	118,243	71.2M	20Mb	2019-01-30

ID:: 6747626

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